Fermentation microbiology and biotechnology pdf


    PDF | Microorganisms, free-living and immobilized, are widely used industrially as catalysts in the biotransformation of many chemical reactions, especially in. PDF | On Dec 12, , Namdar Baghaei-Yazdi and others published Fermentation Microbiology and Biotechnology. Fermentation Microbiology and Biotechnology, Third Edition explores and illustrates the the whole spectrum of fermentation biotechnology, from fermentation kinetics and Chapter 2: Microbiology of Industrial Fermentation · Download PDF.

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    Fermentation Microbiology And Biotechnology Pdf

    International Standard Book Number (eBook - PDF) . Chapter 1 Fermentation Microbiology and Biotechnology: An Historical. Fermentation Microbiology and Biotechnology, Third Edition explores and illustrates the diverse array of DownloadPDF MB Read online. Fermentation Microbiology and Biotechnology PDF - Free download as PDF File .pdf), Text File .txt) or read online for free.

    Fermentation microbiology and biotechnology pdf Fermentation Microbiology and Biotechnology, Third Edition. Fermentation Microbiology and Biotechnology, Third Edition explores and illustrates the diverse array of metabolic pathways employed for the production of. Fermentation Microbiology and Biotechnology - Edited by E. El-Mansi, C. Bryce, A. Damain and A.

    This is an open access article distributed under the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Coffee is one of the most important and widely used commercial crops in the world.

    After ripe coffee cherries are harvested, coffee must pass through several steps to become green raw coffee beans. Commonly, there are three different processing methods used to obtain green coffee beans from coffee cherries, namely, the wet, dry, and semidry methods.

    Fermentation microbiology and biotechnology, 2nd edn

    Microorganisms yeasts and bacteria play a major role in coffee fermentation process by degrading mucilage by producing different enzymes pectinase , acids, and alcohols. Starter culture development is crucial and is done by selecting microorganisms that have certain characteristics, such as mucilage degradation ability, tolerance to stress during fermentation, the ability to suppress the growth of pathogenic fungi, and a positive impact on the sensory quality of the coffee.

    Currently, green coffee beans obtained from farms that use any of the above processing methods are fermented with selected microorganisms to improve the flavour and aroma of the coffee. This is the result of a new insight into the development of unique flavoured coffee and into engaging with the coffee market to better benefit.

    Future prospects are also discussed through the incorporation of recent research. Introduction Coffee is one of the most widely used nonalcoholic drinks, and its consumption is spreading globally.

    It is the second most important commodity exchanged in world markets, next to crude oil. Brazil is the leading producer and exporter of Coffea arabica [ 1 ], followed by Indonesia, Ethiopia, the Philippines, Mexico, Vietnam, and 40 other countries. People drink coffee to relax and enjoy its diverse flavours and aromas.

    It has physiological and psychological effects beyond its nutritional benefits. In recent years, an appreciation for quality coffee has lent importance to specialty coffees, which have unique aromas and remarkable flavours. These characteristics are linked to the areas where the coffees are grown and their cultivated genotypes [ 2 ].

    Some researchers report significant differences among beverages from coffee that is processed using different methods [ 3 , 4 ]. It is currently believed that different processing methods induce different metabolic reactions in coffee fruits, which can affect the chemical compositions of the coffee beans and thereby their cupping quality [ 5 , 6 ].

    Fermentation is a metabolic process that uses sugar, either in the absence anaerobic or presence aerobic of oxygen. Coffee fermentation is critical for removing mucilage from parchment coffee. Coffee mucilage contains polysaccharides pectin , cellulose, and starch. The mucilage can prolong the time needed to dry the coffee beans and, in some cases, also lead to mould development, which reduces the final quality of the coffee.

    The fermentation process is facilitated by enzymes that naturally occur in the coffee fruit and microflora acquired from the environment. Microorganisms yeast, bacteria, and fungi play a major role in degrading mucilage by producing various enzymes, alcohols, and acids during the fermentation process.

    There are several commercially available enzymes for coffee fermentation. The earliest was marketed under the trade name Benefax. Later brands have included Pectozyme, Cofepec, and Ultrazym, which are mixtures of pectinase, hemicellulose, and cellulose [ 7 ]. Current research indicates that coffee fermentation is becoming more popular for producing specialty coffee. Vaast et al. Therefore, the purpose of this review is to briefly explain the need for coffee fermentation and its impact on major coffee quality attributes, in company with recent research outcomes.

    This review will provide a clear understanding of the role of microorganisms in coffee fermentation and can direct future research into commercializing potential starter cultures. Coffee Fermentation Process Fermentation is a chemical process in which complex molecules are broken down into simpler molecules, producing liquid products and gasses volatile compounds. Coffee fruits are processed by one of three methods immediately after harvesting to allow spontaneous or indigenous fermentation to occur.

    The three different coffee processing methods are referred to as dry, wet, and semidry. Depending on the processing type, the length of time needed for fermentation varies. The main purpose of the fermentation process in all methods is to remove the mucilage layer, which is rich in polysaccharides pectin , and to decrease the water content of the coffee beans.

    Wet processing is largely used for Arabica coffee. Immediately after harvesting, the ripe coffee fruits go through a flotation process to clean debris and remove floaters low-density fruits.

    Semidry processing is a combination of both methods, in which the coffee fruits are depulped but the fermentation process occurs directly under the sun on a platform [ 12 ]. Composition of Mucilage Coffee mucilage is a viscous liquid residue produced in the coffee industry that is disposed of without treatment directly into watercourses, causing serious contamination problems.

    The physical and chemical properties of coffee mucilage vary according to species and variety. Mucilage is composed of water Knowing these compositions is essential for the understanding of coffee fermentation. The chemical composition and composition of mucilage before and after fermentation are described in Tables 1 and 2 , respectively. Table 1: Chemical composition, on a wet and dry basis, of coffee mucilage. Table 2: The composition of coffee mucilage before and after complete fermentation.

    Enzymes Produced during Coffee Fermentation Previous reports have identified over 50 yeasts and bacterial species that are present during coffee fermentation [ 15 , 16 ]. Silva et al. Coughlan and Mayer [ 18 ] report that a variety of extracellular enzymes are generated by cellulolytic Bacillus species, which potentially contribute to the breakdown of cellulose and pectin substances that exist in the skin, pulp, and mucilage of coffee cherries [ 11 ].

    The three most important enzymes produced by microorganisms for degrading pectin substances during coffee fermentation are pectin lyase, polygalacturonase, and pectin methyl esterase. Pectin lyase catalyses pectin degradation by trans-elimination, releasing unsaturated galacturonic acids [ 19 ].

    Polygalacturonase is the main enzyme involved in coffee fermentation. Pectin methyl esterase is responsible for the de-esterification of the methoxyl group of the pectin, forming pectic acid and methanol [ 21 ].

    Starter Cultures for Coffee Fermentation A starter culture is a microbiological culture that accelerates the fermentation process. In the food fermentation industry, starter cultures are widely known for use in cheese, yogurt, beer, and wine [ 22 , 23 ].

    The use of starter culture in a controlled coffee fermentation may ensure the best quality of coffee and increase the economic benefits for the growers [ 24 ]. As Corsetti et al. They should also produce sensory quality, prolong shelf life, reduce the processing time, and repress the development of food-related pathogenic microorganisms.

    Only limited studies have been reported on the use of a starter culture for coffee fermentation. The first study, by Agate and Bhat [ 26 ], effectively introduced a coffee fermentation starter culture. This study demonstrated that the addition of a mixture of three Saccharomyces species viz. A study was also conducted on the microbial and physicochemical characteristics of coffee fermentation by inoculating pectinolytic microbes isolated from spontaneous fermentation viz. However, the authors reported that the inoculation of the pectinolytic microbes did not speed up the degradation of the polysaccharide compounds or modify the organoleptic characteristics of the beverage.

    They suggested that it would be better to use lactic acid bacteria instead in order to remain as close as possible to natural fermentation.

    Recently, coffee fermentation using aromatic yeasts to develop flavour has been studied during dry [ 28 ], semidry [ 28 ], and wet [ 29 ] processing. The microbial ecology of the indigenous yeasts from which starter cultures were selected is reported in Tables 3 and 4.

    Table 3: The list of developed culture and their characteristics and effect on coffee fermentation. Table 4: Microbial ecology of yeasts and bacteria of coffee fermentation from which starter culture selected. The Positive Impact of Fermentation on Coffee Flavour and Aroma Around the globe, coffee is popular as a nonalcoholic drink because of its pleasant aroma and refreshing flavours. However, the fermentation process can increase the diversity of coffee aroma and flavour compounds.

    As mentioned by Tressi [ 32 ] and Farah et al. A supporting report indicates that factors during the preharvest and postharvest processes also critically affect the coffee aroma [ 35 ].

    During coffee fermentation, microorganisms produce diverse metabolites.

    Fermentation microbiology and biotechnology, 2nd edn - PDF Free Download

    Microbial activity and the extent of fermentation determine the concentrations of free sugars e. Pederson and Breed [ 37 ] summarized early studies on the implication that fermentation improves coffee quality. Wet-processed coffee has superior aroma qualities to dry-processed coffee because of the aromatic compounds produced during the removal of the mucilage layer in wet processing [ 38 ].

    The selection of appropriate microorganisms that have a positive impact on coffee flavour and aroma during fermentation is critical, and the fermentation process should be controlled to achieve this positive impact. The Negative Impact of Fermentation on Coffee Flavour and Aroma The major challenge in coffee fermentation, according to several studies, is the difficulty of controlling the process.

    Overfermentation is one of several dilemmas described by coffee farmers and scientists [ 39 — 41 ].

    The fermentation process must be well controlled to ensure the development of beneficial microorganisms that produce a high-quality beverage with a good aroma. Underfermented coffee beans contain residual mucilage and sugar that prevent drying and create a conducive environment for the development of spoilage bacteria and fungi. Overfermentation encourages the production of undesirable chemical compounds, notably propionic and butyric acids, which confer off-flavours, such as an onion taste [ 44 — 47 ].

    Lopez et al. Species of the Bacillus genus, especially B. As described by Silva et al. Enterobacteriaceae and acetic acid bacteria lead to the production of excessive acetic acid during prolonged fermentation in dry processing [ 11 ]. Bade-Wegner et al. These can be detrimental to coffee quality if they are present at concentrations higher than 1. Furthermore, the growth of filamentous fungi and mycotoxins, which can generate off-flavours, should be controlled by the management of the fermentation and drying processes [ 48 — 52 ].

    They reported that 24 h yeast fermentation induced a notable increase in antioxidant activity and total polyphenol and flavonoid contents. The consumer acceptance of the fermented coffee beans was slightly lower than that of the controls nonfermented , with the exception of one fermented coffee beans fermented with Saccharomyces species , which gained approximately Rhizopus oligosporus is a common fungal species widely used as a starter culture in fermented foods, such as soy tempeh a traditional fermented soybean product.

    During fermentation by R. A study was conducted on the effect of the solid-state fermentation of green coffee beans by R.

    As reported by Lee et al. Fermentation with R. Coffee enjoys wide global acceptance, and small improvements in coffee quality can make a significant difference and expand market opportunity. Because green coffee beans are used for fermentation instead of parchment coffee with mucilage , this can be done by coffee-processing companies.

    New developments in oxidative fermentation

    However, the isolation and identification of microorganisms that significantly impact coffee sensory quality should be further investigated for the development of a starter culture. Future research may also investigate the commercialization of the aroma and flavour modulation of green coffee beans via fermentation. Conclusion Fermentation is crucial in coffee processing, not only to remove mucilage, but also, if well controlled, to create essential sensory quality characteristics.

    However, it has a disadvantage that if fermentation proceeds beyond the recommended time, microorganisms can also reduce the quality by creating off-flavours and undesirable characteristics.

    Of the diverse microflora found in natural coffee fermentation, a few may be selected for use as a starter culture. However, the potential use of starter cultures for coffee fermentation is not yet well studied. The selection of food-grade microorganisms and their use in fermenting green coffee beans require further research.

    This review provides precise information about the role of microorganisms in coffee fermentation and their impact on coffee flavour and aroma. Other molecular sciences can contribute to an understanding of the chemical compounds produced during fermentation and their impact on coffee quality, leading to the availability of more credible and advanced information. Integrating multidisciplinary research will be helpful in more detailed studies.

    Conflicts of Interest The authors declare that they have no conflicts of interest. Acknowledgments This study was supported by research grant from the Kangwon National University no. Taveira, F. Figueiredo et al. Selmar, G. Bytof, S. Knopp, and B. Leloup, C. Gancel, R. Liardon, A. Rytz, and A.

    Knopp, P. Schieberle, I. Teutsch, and D. Knopp, D. Kramer et al. Schwan and G. Vaast, B. Bertrand, J.

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    No abstract is available for this article. Citing Literature Number of times cited according to CrossRef: Levin and Nuri Azbar , Cell immobilization for microbial production of 1,3-propanediol , Critical Reviews in Biotechnology ,

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